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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10545 |
| Description | Anti-TIGAR Antibody |
| Alternate Names |
|
| Background Information | TIGAR (TP53-induced glycolysis and apoptosis regulator) is a p53-inducible protein that is thought to diminish levels of reactive oxygen species (ROS) and this may also contribute to p53 protecting from genomic damage. TIGAR’s ability to lower ROS levels may also have an important role to play in the autophagic response. It is known to decrease levels of fructose-2,6-bisphosphate and in turn inhibits glycolysis. Under ischemic stress, recent research has shown that TIGAR and p53 can regulate cellular energy homeostasis and apoptosis. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-TIGAR Antibody is an antibody against TIGAR for use in WB, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected TIGAR in normal human brain and glioblastoma cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human TIGAR. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human, mouse, and rat. Predicted to react with bovine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Probable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by TP53/p53. CATALYTIC ACTIVITY: Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. INDUCTION: Rapidly up-regulated by TP53/p53. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SEQUENCE SIMILARITIES: Belongs to the phosphoglycerate mutase family. CAUTION: Not expected to have any kinase activity. |
| Molecular Weight | ~30 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected TIGAR in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |