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Millipore This Anti-Tryptophan Hydroxylase 1/Tph1 Antibody Is Validated For Use In Western Blotting & Ihc (Paraffin) For The Detection Of Tryptophan Hydroxylase 1/Tph1. - Mill (Additional S&H or Hazmat Fees May Apply)
Tryptophan hydroxylase 1/TPH1, also known as Tryptophan 5-hydroxylase 1, also known as Tryptophan 5-monooxygenase 1, and encoded by the gene TPH1/TPH/TPRH/TRPH, is an important biosynthetic enzyme needed for serotonin synthesis from L-trytophan. TPH1 is responsible for the addition of the -OH grouip to 5-hydroxytryptophan which is the initial and rate limiting step in the synthesis of serotonin. Serotonin in a neurotransmitter and has an antidepressant effect on the brain. The activity of TPH1 is also modulated by phosphorylation Protein Kinase A is responsible for the phosphorylation of TPH1 and helps to modulate its activity in neurons. TPH1 is mostly expressed in tissues that express serotonin such as the brain, pineal gland, and CNS but also can be found in skin and intestinal tissues.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Tryptophan hydroxylase 1/TPH1 antibody is validated for use in western blotting & IHC (Paraffin) for the detection of Tryptophan hydroxylase 1/TPH1.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50-500 dilution from a representative lot detected Tryptophan hydroxylase 1/TPH1 in mouse pancreas and mouse colon tissue. Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Tryptophan hydroxylase 1/TPH1 in mouse cerebellum and mouse cerebral cortex tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of rat Tryptophan hydroxylase 1/TPH1.
Epitope
C-terminus
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Mouse
Species Reactivity Note
Mouse. Predicted to react with Rat based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001094104
Gene Symbol
Tph1
Tph
Purification Method
Affinity Purfied
UniProt Number
P09810
Molecular Weight
~51 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in P815 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Tryptophan hydroxylase 1/TPH1 in 10 µg of P815 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
