Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M | WB, IH(P), PIA | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB15858 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Aquaporin 5 Antibody |
| Alternate Names |
|
| Background Information | Aquaporin-5 (UniProt: P47864; also known as AQP-5) is encoded by the Aqp gene (Gene ID: 25241) in rat. Aquaporins, a highly conserved group of membrane proteins, are involved in the bidirectional transfer of osmotically driven water and small solutes across cell membranes. They contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). Aquaporin-5 is a homotetrameric, multi-pass membrane glycoprotein that contains five cytoplasmic domains, six continuous transmembrane domains, two discontinuous transmembrane domains, and four extracellular domains. It is detected in salivary glands, lacrimal glands, corneal epithelium in eye, trachea, and lung. Its NPA motifs are localized in amino acids 69-71 and 185-187. It plays an important role in fluid secretion in salivary glands and is shown to be essential for transient receptor potential cation channel subfamily V member 4) (TRPV4) activation by hypotonicity. Together with TRPV4 it controls regulatory volume decrease in salivary epithelial cells. Mutations in AQP5 gene have been linked to the development of palmoplantar keratoderma, a disease that is characterized by an abnormal thickening of the skin on the palms of the hands and soles of feet. Overexpression of Aquaporin-5 has also been observed in several human cancers and knockdown of AQP5 leads to partially reduced cell cycle progression from G1 to S phase and apoptosis. (Ref.: Direito, I., et al. (2016). Cell. Mol. Life Sci. 73(8); 1623-1640; Raina, S., et al. (1995). J. Biol. Chem. 270(4); 1908-1912). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Aquaporin 5, Cat. No. AB15858, is a rabbit polyclonal antibody that detects Aquaporin 5 and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested applications Peptide Inhibition Assay: Target band detection in lysate from Rat salivary gland tissue was prevented by preblocking of a representative lot with the immunogen peptide. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Aquaporin 5 in Rat lung and Mouse trachea tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of rat Aquaporin 5. |
| Epitope | Cytoplasmic domain |
| Concentration | 1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects Aquaporin 5. It targets an epitope within 17 amino acids within the cytoplasmic domain from the C-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse, Rat |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. |
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P55064 # Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. SIZE: 265 amino acids; 28292 Da SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. DOMAIN: SwissProt: P55064 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. |
| Molecular Weight | ~22 kDa observed; 28.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Rat salivary gland tissue lysate. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Aquaporin 5 in Rat salivary gland tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |