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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB, IH(P), ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2290 |
| Description | Anti-MAP-2 Antibody |
| Alternate Names |
|
| Background Information | Microtubule-associated protein 2 (UniProt: P15146; also known as MAP-2) is encoded by the Map2 (also known as Mtap2) gene (Gene ID: 25595) in rat. MAP-2 belong to the family of thermostable proteins associated with microtubules. It is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules against depolymerization. It also has a stiffening effect on microtubules. MAP-2 is shown to be essential for the development and maintenance of neuronal morphology. Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene) have been identified in neurons. The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation. All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif. MAP-2 can be phosphorylated at serine residues in K-X-g-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2) that causes its detachment from microtubules and results in their disassembly. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MAP-2 Antibody, Cat. No. AB2290, is a highly specific rabbit polyclonal antibody that targets rat MAP-2 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in E18 rat hippocampus neurons. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in mouse cerebellum, rat cerebral cortex, and rat kidney tissues. |
| Biological Information | |
|---|---|
| Immunogen | Three KLH-conjugated linear peptides corresponding to the N-terminal and central regions of rat MAP-2 . |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes MAP-2. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with rat. Other homologies: Mouse (94% sequence homology). Human (72% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Regulates and organizes microtubules; may serve as a scaffolding protein for neuromodulatory activities [RGD]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~270 kDa observed; 202.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain microsomal preparation. Western Blotting Analysis: 1 µg/mL of this antibody detected MAP-2 in 10 µg of rat brain microsomal preparation. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |