Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3256-I |
| Description | Anti-Luciferase |
| Alternate Names |
|
| Background Information | Luciferin 4-monooxygenase (UniProt: P08659; also known as EC: 1.13.12.7, Luciferase) is a peroxisomal enzyme that belongs to the ATP-dependent AMP-binding enzyme family. It catalyzes the production of oxyluciferin from luciferin in an ATP and magnesium dependent reaction and releases a photon of light when oxyluciferin returns to the ground state. This reaction proceeds in two steps where the first step involves adenylation of luciferin and in the second step luciferase acts as an oxygenase on adenyl-luciferin to produce oxyluciferin and carbon dioxide, the decay of oxyluciferin producing a photon of light of 562 nm. Luciferase from the firefly is commonly used in reporter assay where the total light emission is proportional to the luciferase activity of the sample. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Luciferase, Cat. No. AB3256-I, is a rabbit polyclonal antibody that detects firefly luciferase and has been tested for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Luciferase in HeLa cells transfected with Luciferase expression plasmid. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged full length recombinant firefly luciferase. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects firefly luciferase. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | All |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
|
| Molecular Weight | ~58 kDa observed; 60.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in lysate from HeLa cells transfected with Luciferase expression plasmid. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Luciferase in HeLa cells transfected with luciferase expression plasmid. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |