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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB4330 |
| Description | Anti-DDX4 (Vasa) Antibody |
| Alternate Names |
|
| Background Information | DDX4 (Vasa or MVH) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. DDX4 (Vasa) proteins are mostly localized in testicular germ cells in the cytoplasm of spermatocytes. DDX4 (Vasa) is a high-copy extragenic suppressor gene. DDX4 (Vasa) is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected DDX4 (Vasa) in normal human testis tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminus of mouse DDX4 (Vasa). |
| Epitope | C-terminus |
| Host | Rabbit |
| Specificity | This antibody recognizes DDX4 (Vasa) at the C-terminus. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: May play a role in germ cell development. CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBUNIT STRUCTURE: Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Ref.3 SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › perinuclear region. TISSUE SPECIFICTY: Testis. DEVELOPMENTAL STAGE: Expressed in spermatogenic cells from the spermatocyte stage to the round spermatid stage. SEQUENCE SIMILARITIES: Belongs to the DEAD box helicase family. DDX4/VASA subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. |
| Molecular Weight | ~76 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse testis tissue lysate. Western Blot Analysis: A 1:500 dilution of this antibody detected DDX4 (Vasa) in 10 µg of mouse testis tissue lysate. Western Blot performed without Tween. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |