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| Description | |
|---|---|
| Catalogue Number | AB5603-25UG |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Sox2 (rabbit polyclonal) |
| Alternate Names |
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| Background Information | Transcription factor SOX-2 (UniProt P48431; also known as Sex determining region Y-box 2, SRY-related HMG-box gene 2, Transcription factor SOX2) is encoded by the SOX2 (also known as ANOP3, MCOPS3) gene (Gene ID 6657) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences coding the HMG-box domains that mediate sequence-specific DNA binding. Sox genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of SOX genes cluster at at least 40 genomic loci and at least 30 SOX genes have been identified. Sox2 plays an essential role in maintaining the pluripotency and self-renewal of undifferentiated embryonic stem cells. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Sox2 Antibody is an antibody against Sox2 for use in IC & WB with more than 60 product citations. |
| Key Applications |
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| Application Notes | Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Sox2 in H9 human stem cells. Immunocytochemistry Analysis: A representative lot detected nuclear Sox2 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human embryonic stem cells (hESCs) by fluorescent immunocytochemistry (Kingham, E., et al. (2013). Small. 9(12):2140-2151). Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed iPSCs generated from human kidney tubular renal epithelial cells (Montserrat, N., et al. (2012). J. Biol. Chem. 287(29):24131-24138). Immunocytochemistry Analysis: A representative lot detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized iPSCs generated from the skin cells of patients with Parkinsons disease (Sánchez-Danés, A., et al. (2012). EMBO Mol. Med. 4(5):380-395). Immunocytochemistry Analysis: Representative lots detected Sox2 immunoreactivity by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized iPSCs generated from mouse ear cells and MEF (Burns, J.C., et al. (2012). PLoS One. 7(10):e48704; Hadadeh, O., et al. (2012). PLoS One. 7(11):e49065). Western Blotting Analysis: A representative lot detected Sox2 in mouse embryonic stem cell (mESC) nuclear extracts (Banaszynski, L.A., et al. (2013). Cell. 155(1):107-120). Immunofluorescence Analysis: A representative lot detected Sox2-positive cells in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized mouse brain vibratome sections by fluorescent immunohistochemistry (Matsuda, S., et al. (2012). J. Neurosci. 32(36):12543-12557). Immunohistochemistry Analysis: A representative lot detected Sox2 immunoreactivity in 4% paraformaldehyde-fixed whole mount mouse embryo sections (van Rooijen, C., et al. Development. 139(14):2576-2583). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human Sox2. |
| Epitope | C-terminal region. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This antibody recognizes Sox2 C-terminal region. |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. Predicted to react with Rat, Bovine, Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~39 kDa observed. 34.31 kDa (human) and 34.45 (mouse) calculated. Uncharacterized band(s) may appear in some lysates |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in mESC lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Sox2 in 10 µg of mESC lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
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| Material Size | 25 μg |