Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, B | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5610-I-25UG |
| Description | Anti-GABA A Receptor alpha 6 |
| Alternate Names |
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| Background Information | Gamma-aminobutyric acid type A receptor alpha 6 subunit (UniProt: E1BE96; also known as GABA A receptor alpha 6) is encoded by the GABRA6 gene (Gene ID: 539194) in bovine. GABA is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA is produced from glutamic acid in a reaction catalyzed by glutamic acid decarboxylase. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. They differ in their pharmacological, electrophysiological, and biochemical properties. GABAA-receptor are pentameric membrane proteins that mediate an increase in membrane conductance with an equilibrium potential near the resting level of 70 mV. This conductance increase often is accompanied by a membrane hyperpolarization, which later results in a reduction in the probability of action potential initiation. The reduction in membrane resistance is accomplished by the GABA-dependent facilitation of chloride ion influx. Mos GABA A receptors contain one type of alpha and beta subunit, and a single gamma polypeptide in a ratio of 2:2:1. GABA A receptor alpha 6 subunit is synthesized with a signal peptide (aa 1-19), which is cleaved off in the mature form. It contains four transmembrane domains (aa 240-262, 274-293, 305-328, and 423-441). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-GABA A Receptor alpha 6, Cat. No. AB5610-I, is a highly specific rabbit polyclonal antibody that targets Gamma-aminobutyric acid type A receptor alpha6 subunit and has been tested in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected GABA A Receptor alpha 6 in mouse cerebellum tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of bovine GABA A receptor alpha 6 subunit. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects GABA A receptor alpha 6 subunit. It targets an epitope with in 16 amino acids from the N-terminal region. |
| Species Reactivity |
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| Species Reactivity Note | Bovine, Rat. Predicted to react with Mouse, Human based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~51 kDa observed; 51.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in bovine brain membrane extract. Western Blotting Analysis: 1 µg/mL of this antibody detected GABA A Receptor alpha 6 in bovine brain membrane extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |