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Millipore Anti-Dab1, Cat. No. Ab5840-I, Is A Highly Specific Rabbit Polyclonal Antibody That Targets Disabled Homolog 1 And Has Been Tested In Immunohistochemistry (Paraffin) And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Disabled homolog 1 (UniProt: P97318; also known as DAB1) is encoded by the Dab1 gene (Gene ID: 13131) in murine species. Dab 1 is mainly expressed in the brain and serves as an adapter molecule involved in neural development where it is reported to direct the migration of cortical neurons past previously formed neurons to reach their proper layer. DAB1 is can be phosphorylated on Tyr198, Tyr220, and Tyr232 upon reelin induction in embryonic neurons. It can also be phosphorylated on Ser524, but this phosphorylation is independent of reelin signaling. Tyrosine phosphorylation of DAB1 by Src family tyrosine kinases is considered to be the most critical downstream event in Reelin signaling. DAB1 contains a phosphotyrosine interaction domain (PID) (aa 36-189), which specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins. Seven different isoforms of Dab1 have been identified that are generated via alternative splicing.
Product Information
Format
Purified
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-Dab1, Cat. No. AB5840-I, is a highly specific rabbit polyclonal antibody that targets Disabled homolog 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Dab1 in mouse cerebral cortex tissue sections.
Biological Information
Immunogen
GST-tagged recombinant fragment corresponding to 156 amino acids from the C-terminal half of murine Disabled homolog 1 (Dab1).
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects murine Disabled homolog 1 (Dab1). It targets an epitope within 156 amino acids from the C-terminal half.
Species Reactivity
Mouse
Species Reactivity Note
Mouse.
Antibody Type
Polyclonal Antibody
Gene Symbol
Dab1
Purification Method
Affinity Purified
UniProt Number
P97318
Molecular Weight
~64 kDa observed; 63.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse brain tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected Dab1 in 10 µg of m ouse brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
