Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC1389-25UL |
| Description | Anti-DCLK1-S |
| Alternate Names |
|
| Background Information | Serine/threonine-protein kinase DCLK1 (UniProt: O15075; also known as EC: 2.7.11.1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1) is encoded by the DCLK1 (also known as DCAMKL1, DCDC3A, KIAA0369) gene (Gene ID: 9201) in human. DCLK1 is an integral component of plasma membrane and is highly expressed in fetal brain tissue and is also detectable in lung and liver. Adults show ubiquitous expression. It has two doublecortin domains (aa 57-143 and 186-269). Four isoforms of DCLK1 have been reported (in UniProt) that are produced by alternative splicing. DCLK1 expression is reported to be critical for growth of human colon cancer cells and its expression is up-regulated in response to growth factors resulting in hyperproliferation. A short isoform, DCLK1-S, has been reported in human colorectal cancer cells and in cancer stem cells, while normal colon cells express only the long isoform of DCLK1. Hence, DCLK1-S may serve as a specific marker for cancer stem cells and the long isoform indicates normal human cells. The short isoform displays about 98% sequence homology with the C-terminal domains of the long isoform, but differs from it by six amino acids at the N-terminal end. Higher expression of Dclk-1s has been linked to poor prognosis and survival rate in colorectal cancer subjects. (Ref.: Sarkar, S et al. (2017). Lab. Invest. doi:10. 1038/labinvest.2017.40). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-DCLK1-S, Cat. No. ABC1389, is a rabbit polyclonal antibody that specifically detects the short version of Serine/threonine-protein kinase DCLK1. It has been tested for use in Immunohistochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot detected DCLK1-S in Immunohistochemistry applications (Sarkar, S., et. al. (2017). Lab Invest. [Epub ahead of print]). Western Blotting Analysis: A representative lot detected DCLK1-S in Western Blotting applications (Sarkar, S., et. al. (2017). Lab Invest. [Epub ahead of print]). |
| Biological Information | |
|---|---|
| Immunogen | A multimeric scaffold of the 6 amino acids from the N-terminal region of the short version of human DCLK1 (DCLK1-S). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects the short version of Serine/threonine-protein kinase DCLK1 (DCLK1-S). It does not react with long isoforms. It targets an epitope within six amino acids from the N-terminus region. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~55 kDa observed. 47.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HCT116 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected DCLK1-S in 10 µg of HCT116 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |