Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IH(P), IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC1454 |
| Description | Anti-Sodium-Iodide Symporter Antibody, C-terminal 2 |
| Alternate Names |
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| Background Information | Sodium/iodide cotransporter (UniProt: Q92911; also known as Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter) is encoded by the SLC5A5 (also known as NIS) gene (Gene ID: 6528) in human. Sodium/iodide cotransporter is a multi-pass membrane protein that mediates iodide uptake in thyroid gland. It is expressed primarily in thyroid tissue, but is present in lower levels in mammary gland and ovary. It expression is significantly reduced in tumors. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. NIS gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Abnormalities in the expression of NIS gene lead to thyroid disease and can result in congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 76 mM Tris, 192 mM glycine, pH 7.4 with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Sodium-Iodide Symporter, C-terminal 2, Cat. No. ABC1454, is a rabbit polyclonal antibody that detects Sodium/iodide cotransporter and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Sodium-Iodide Symporter in human thyroid and human salivary gland tissue sections. Immunocytochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with wild-type human NIS and in COS-7 cells transfected with mutant Q267E human NIS (De la Vieja, A., et. al. (2005). Mol Endocrinol. 19(11):2847-58; De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87). Immunohistochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in Immunohistochemistry application (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87; Wapnir, I.L., et. al. (2004). Clin Cancer Res. 10(13):4294-302; Wapnir, I.L., et. al. (2003). J Clin Endocrinol Metab. 88(4):1880-8). Immunoprecipitation Analysis: A representative lot detected Sodium-Iodide Symporter in Biotinylated NIS constructs expressed in COS-7 cells (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87). Flow Cytometry Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with WT and mutant Q267E NIS (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87). Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with hNIS (Courtesy of Dr Nancy Carrasco & Dr. Andrea Reyna-Neyra at Yale University). Western Blotting Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with NIS and COS-7 cells transfected with WT and mutant Q267E NIS (De la Vieja, A., et. al. (2005). Mol Endocrinol. 19(11):2847-58; De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87). Immunohistochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in IHC-P human salivary gland and breast tissues (Tazebay, U.H., et. al. (2000). Nat Med. 6(8):871-8). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Sodium-iodide symporter. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Sodium-iodide symporter in human. It targets an epitope within 13 amino acids from the C-terminal end. For Western blotting application, please do not boil the sample to avoid any aggregation. When transfering protein to nitrocellulose membrane use of 0.05% (w/v) lithium dodecyl sufate is highly recommended in the transfer buffer. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | 68.66 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human stomach tissue sections. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sodium-Iodide Symporter in human stomach tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |