| Description | |
|---|---|
| Catalogue Number | ABC1688-100UG |
| Description | Anti-SWAP-70 |
| Alternate Names |
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| Background Information | Switch-associated protein 70 (UniProt: Q9UH65; also known as SWAP-70) is encoded by the SWAP70 (also known as KIAA0640, HSPC321) gene (Gene ID: 23075) in human. SWAP-70 is a phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent guanine nucleotide exchange factor (GEF) that, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. SWAP-70 regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. It is expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG, but not IgM. It is also widely expressed in spleen, kidney, lung, and liver. SWAP-70 has an N-terminal putative EF-hand domain, a pleckstrin homology (PH) domain (aa 210-306) that binds phosphatidylinositol 3,4,5-trisphosphate and is responsible for membrane localization, a tri-partite coiled-coil region (aa 316-539), and a C-terminal F-actin-binding site through which it specifically binds non-muscle actin. (Ref.: Chacón-Martínez, CA et al. (2103). J. Biol. Chem. 288(40); 28687-28703). |
| Product Information | |
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| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
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| Application | Anti-SWAP-70, Cat. No. ABC1688, is a rabbit polyclonal antibody that detects Switch-associated protein 70 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
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| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected SWAP-70 in human tonsil and human lung tissue sections. |
| Biological Information | |
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| Immunogen | KLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal region of human Switch-associated protein 70. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects human Switch-associated protein 70. It targets an epitope with in 12 amino acids from the N-terminal region. |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Bovine, Mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~70 kDa observed; 69.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Western Blotting in Raji cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected SWAP-70 in Raji cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 100 μg |