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Millipore This Anti-Phospho Usp7 (Ser18) Antibody Is Validated For Use In Western Blotting & Peptide Inhibition Assay For The Detection Of Phospho Usp7 (Ser18). - Mill (Additional S&H or Hazmat Fees May Apply)
Ubiquitin-specific-processing protease 7 (USP7) is also known as Ubiquitin carboxyl-terminal hydrolase 7 or Herpesvirus-associated ubiquitin-specific protease (HAUSP). USP7 is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, USP7 activity generally stabilizes its substrate proteins, and it is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-phospho USP7 (Ser18) antibody is validated for use in western blotting & peptide inhibition assay for the detection of phospho USP7 (Ser18).
Key Applications
Western Blotting
Peptide Inhibition Assay
Application Notes
Peptide Inhibition Analysis: 0.2 µg/mL from a representative lot was blocked by a phospho-peptide in HeLa cell lysate.
Western Blotting Analysis: 0.02 µg/mL from a representative lot detected phospho USP7 (Ser18) in HeLa cell lysate (Prof. Grigory Dianov, University of Oxford, U.K.).
Western Blotting Analysis: 0.02 µg/mL from a representative lot detected 100 ng of phospho USP7 (Ser18), and not dephosphorylated USP7 (Prof. Grigory Dianov, University of Oxford, U.K.).
Alexa Fluor® is a registered trademark of Life Technologies®.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to a region near the N-terminus of human USP7 phosphorylated at Ser18.
Epitope
Near N-terminus
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Mouse, Rat, Porcine, and Xenopus based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_003461
Gene Symbol
USP7
HAUSP
Modifications
Phosphorylation
Purification Method
Affinity Purfied
UniProt Number
Q93009
Molecular Weight
~140 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected phospho USP7 (Ser18) in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
