Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC253 |
| Description | Anti-Beclin-2 |
| Alternate Names |
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| Background Information | Beclin-2 (UniProt: P0DM65) is encoded by Becn2 gene (Gene ID: 226720) in murine species. Beclin-2 is a member of the Atg6/Beclin family and is involved in 2 distinct lysosomal degradation pathways and acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. It regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1. Beclin-2 family members are specific to mammals whereas Becln-1 subfamily members are present in more primitive vertebrates. Beclin-2 interacts with ATG14, AMBRA1, UVRAG and PIK3C3/VPS34 and this interaction is not disrupted by starvation. It is expressed in brain, skeletal muscle, placenta, thymus and uterus and expression is lower in liver, testis, stomach, and 17-day-old embryos. Disruption of Beclin-2 is shown to reduce embryonic viability and heterozygous knockout mice show defects in autophagy, increased levels of Cnr1 receptor, elevated food intake, and obesity and insulin resistance. (Ref.: He, C. et al. (2013). Cell 154, 1085-1099). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Beclin-2 Antibody, Cat. No. ABC253, is a rabbit polyclonal antibdoy that detects Beclin-2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Beclin-2 in human brain (thalamic region) and mouse stomach tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of murine Beclin-2. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Becline-2 in murine cells. It targets an epitope within 14 amino acids from the N-terminal. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Mouse, Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~50 kDa observed; 50.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Beclin2 +/+ Mouse Brain (Wild-Type). Western Blotting Analysis: 2 µg/mL of this antibody detected Beclin-2 in lysate from Beclin-2 +/+ Mouse Brain (Wild-type). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |