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Pyruvate kinase isozymes M1/M2 (PKM2) is also called Cytosolic thyroid hormone-binding protein (CTHBP), Opa-interacting protein 3 (OIP-3), Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1 (THBP1) and p58. PKM2 is a glycolytic enzyme that assists in generating ATP. PKM2 also stimulates POU5F1-mediated transcriptional activation, and plays a role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. This control of glycolysis and is important for tumor cell proliferation and survival. PKM2 is specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, and cancer cells.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-PKM1 Antibody detects level of PKM1 & has been published & validated for use in PKM1.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PKM1 in 10 µg of mouse brain and RAW264.7 tissue lysate. Western Blotting Analysis: A representative lot detected PKM1 in HEK293 cell lysate (Christofk, R. H., et Al. (2008). Nature. 452:230-233). Immunohistochemistry Analysis: A representative lot detected PKM1 in HEK293 tissue (Christofk, R. H., et Al. (2008). Nature. 452:230-233).
Biological Information
Immunogen
Linear peptide corresponding to human PKM1.
Host
Rabbit
Specificity
This anitbody is specific to PKM1.
Species Reactivity
Mouse
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001193725
Gene Symbol
PKM
OIP3
PK2
PK3
PKM2
Purification Method
Unpurified
UniProt Number
P14618
Molecular Weight
~58 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in C2C12 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PKM1 in 10 µg of C2C12 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
