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Millipore This Anti-Kallikrein-5 Antibody/Klk5 Is Validated For Use In Western Blotting And Immunohistochemistry For The Detection Of Kallikrein-5/Klk5. - Mill (Additional S&H or Hazmat Fees May Apply)
Kallikrein-5/KLK5, or also known as Kallikrein-related peptidase 5, Kallikrein-like protein 2, KLK-L2, and formerly known as Stratum corneum tryptic enzyme (SCTE), is an important enzyme encoded by the gene KLK5/KLK-L2/SCTE. KLK5 is a secreted serine protease enzyme that is expressed in many tissues including the skin, breast and brain. In the skin, Kallikreins-related peptidases (KLKs) are proteases that are important for the desquamation process of the skin (cell shedding). KLK5 is considered as one of the most important KLKs in skin desquamation because KLK5 also regulates the activation of other Kallikreins needed for cell shedding. In disease, KLK5 displays aberrant expression in many cancers. However, expression of KLK5 in some cancer cells appears to suppress tumor growth and activity via reduced RhoA activity and the modulation the mevalonate pathway. This is particularly true in breast cancer and efforts are examining methods to increase KLK5 expression in breast cancer as a form of treatment.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Kallikrein-5 Antibody/KLK5 is validated for use in Western Blotting and Immunohistochemistry for the detection of Kallikrein-5/KLK5.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Kallikrein-5/KLK5 in human skin cancer sections tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the Peptidase S1 domain of human Kallikrein-5/KLK5.
Epitope
Peptidase S1 domain
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Mouse
Species Reactivity Note
Human, Mouse. Predicted to react with Chimpanzee, Gorilla based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001070959
Gene Symbol
KLK5
SCTE
Purification Method
Affinity Purfied
UniProt Number
Q9Y337
Molecular Weight
~32 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in ovcar 3 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Kallikrein-5/KLK5 in 10 µg of ovcar 3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
