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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC474 |
| Replaces | MABS405 |
| Description | Anti-Fhl1 Antibody |
| Alternate Names |
|
| Background Information | Four and a half LIM domains protein 1 (FHL1) is also called Skeletal muscle LIM-protein 1 (SLIM-1). FHL1 has 3 isoforms; Isoform 1 is highly expressed in skeletal muscle; Isoform 2 is expressed in brain and skeletal muscle; and Isoform 3 is expressed in testis, heart and skeletal muscle. FHL1 is possibly involved in muscle development or hypertrophy and associated with X-linked dominant scapuloperoneal myopathy (SPM), X-linked myopathy with postural muscle atrophy (XMPMA), X-linked severe early-onset reducing body myopathy (RBM) and X-linked childhood-onset reducing body myopathy (CO-RBM). |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal in buffer containing serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Four & a half LIM domains protein 1 using this rabbit polyclonal antibody, Anti-Fhl1 Antibody validated for use in western blotting, ICC & IHC. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Fhl1 in a panel of select tissue lysates. Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Fhl1 in an additional panel of select tissue lysates. Western Blotting Analysis: A representative lot from an independent laboratory detected Fhl1 in nonanchored and anchored CasKO and Cx43KO cell lysates. A representative lot from the same independent laboratory detected Fhl1 in non-SRC transformed Cx43KO cell lysate and not in SRC transformed Cx43KO cell lysate (Shen, Y., et Al. (2006). Cancer Res. 66(3):1543-1552.). Western Blotting Analysis: A representative lot from an independent laboratory detected Fhl1 in non-SRC transformed Cx43KO cell lysate and not in SRC transformed Cx43KO cell lysate (Li, X., et Al. (2008). Cancer Sci. 99(7):1326-1333.). Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Fhl1 in normal brain, kidney, liver, lung, prostate, and skin tissues and demonstrated a loss of signal in breast carcinoma, renal carcinoma, hepatocarcinoma, pulmonary carcinoma, prostatic carcinoma, and melanoma tissues (Shen, Y., et Al. (2006). Cancer Res. 66(3):1543-1552.). Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Fhl1 in normal epithelium of breast, kidney, and prostate tissues, and demonstrated a loss of signal in mammary duct carcinoma, renal cell carcinoma, and prostate adenocarcinoma tissues (Li, X., et Al. (2008). Cancer Sci. 99(7):1326-1333.). Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Fhl1 in non SRC transformed Cx43KO and CasKO cells and demonstrated a loss of signal in SRC transformed Cx43KO and CasKO cells (Shen, Y., et Al. (2006). Cancer Res. 66(3):1543-1552.). |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to amino acid sequence CRDPLQGKKYVQKDGRH of human Fhl1. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~32 kDa observed. The calculated molecular weight of isoform 1 for this protein is 32 kDa; however, a dimer may be observed at ~64 kDa in some cell lysates. Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human brain tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Fhl1 in 10 µg of human brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |