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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC513 |
| Description | Anti-Rab11-FIP1/RCP Antibody |
| Alternate Names |
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| Background Information | The protein Rab11-FIP1/RCP, or alternatively named Rab11 family-interacting protein 1, or Rab-coupling protein, and encoded by the human gene RAB11FIP1/RCP is an effector protein that interacts with Rab11 in the processes surrounding endosomal recycling. Rab11-FIP1/RCP also is involved in membrane trafficking in phagocytosis, as well as during axon growth, mediating integrin trafficking in various cancers. RAB11-FIP1/RCP has isoforms. Isoform 1 is non-membrane bound and is localized in the endosome and is involved in endosome recycling. Isoform 2 is more cytoplasmic being confined, for example, to the cytoplasmic phagosome vesicles and the phagosome membrane. Rab11-FIP1/RCP is expressed in multiple tissues and comprises one member of a large family of Rab11 family interacting proteins (Fab11-FIPs) that define discrete subdomains and carry out temporally distinct roles within the recycling and membrane trafficking system of the cell. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Rab11-FIP1/RCP Antibody is validated for use in western blotting, ICC & IP for the detection of Rab11-FIP1/RCP. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab11-FIP1/RCP in 10 µg of C2C12, HEK293, HeLa, HepG2, HUVEC, Jurkat, L6, NIH/3T3, PC-12, PC-3, RAW264.7 cell lysate. Western Blotting Analysis: A representative lot from an independent laboratory detected Rab11-FIP1/RCP in Tac-TGN38 expressing HeLa cell lysates (Jing, J., et Al. (2010). Mol Cell Biol. 21(17):3041-3053.). Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Rab11-FIP1/RCP in Tac-TGN38 expressing HeLa cells (Jing, J., et Al. (2010). Mol Cell Biol. 21(17):3041-3053.). Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Rab11-FIP1/RCP from HeLa cell lysate (Jing, J., et Al. (2010). Mol Cell Biol. 21(17):3041-3053.). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human Rab11-FIP1/RCP. |
| Host | Rabbit |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~90 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab11-FIP1/RCP in 10 µg of A431 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |