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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC, IF | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC822 |
| Description | Anti-LIMP II Antibody |
| Alternate Names |
|
| Background Information | Lysosome membrane protein 2, also known as 85 kDa lysosomal membrane sialoglycoprotein (LGP85) or Lysosome membrane protein II (LIMP II) or Scavenger receptor class B member 2, and encoded by the gene name SCARB2 or CD36L2 or LIMPII, is a heavily glycosylated type III transmembrane protein, the majority of which exists in the lumen of the lysosome and a cytoplasmic domain of approximately 20 amino acids. A deficiency of LIMP II in mice causes uretic pelvic junction obstruction, deafness, and peripheral neuropathy associated with impaired vesicular trafficking and distribution of apically expressed proteins. More recently, LIMP II was shown to act as a receptor to bind β-glucocerebrosidase, the enzyme defective in Gaucher disease, a lysosomal storage disorder. LIMP II-deficient mice showed missorted as well as secreted β-glucocerebrosidase, suggesting that LIMP II also functions as the mannose-6-phosphate-independent trafficking receptor. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-LIMP II Antibody is an antibody against LIMP II for use in western blotting, IHC & immunofluorescence. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 2.5 μg/mL from a representative lot detected LIMP II in human skeletal muscle tissue. Immunofluorescence Analysis: 20 μg/mL from a representative lot detected LIMP II in human skeletal muscle cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated synthetic peptide corresponding to the center region of human LIMP II. |
| Epitope | center region |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Antigen Affinity Purified |
| UniProt Number |
|
| Molecular Weight | ~80 kDa observed. Despite its predicted molecular weight, LIMP II runs at approximately 80 – 85 kDa in Western Blotting. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human skeletal muscle tissue lysate with and without blocking peptide. Western Blotting Analysis: 1 μg/mL of this antibody detected GPAT-1 in human skeletal muscle tissue lysate with and without blocking peptide. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |