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Nucleolar protein 3 (NOL3), also known as Apoptosis repressor with CARD or Muscle-enriched cytoplasmic protein (Myp) or Nucleolar protein of 30 kDa (Nop30) and encoded by the gene name NOL3 or ARC contains one CARD domain. Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. ARC interacts with caspase-2 and –8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-ARC Antibody detects level of ARC & has been published & validated for use in ARC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: 5 μg/mL from a representative lot detected ARC in human heart and skeletal muscle tissues.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to amino acids 191-208 at the C-terminus of human ARC.
Epitope
C-terminus
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Gene Symbol
NOL3
ARC
NOP
Purification Method
Antigen Affinity Purified
UniProt Number
O60936
Molecular Weight
~24 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa, KB, and A549 cell lysates.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARC in HeLa, KB, and A549 cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
