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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABC936 |
| Description | Anti-RINT-1 Antibody |
| Alternate Names |
|
| Background Information | RAD50-interacting protein 1 (UniProt Q6NUQ1; also known as HsRINT-1, RINT-1, RAD50 interactor 1) is encoded by the RINT1 gene (Gene ID 60561) in human. Originally identified and named as a RAD50-interacting partner by a yeast two-hybrid screening, RINT1 is involved in the G2/M checkpoint, maintenance of centrosome integrity, telomere elongation, Golgi apparatus, Golgi-ER vesicular trafficking and Cis- and Trans-Golgi homeostasis. Rint1 homozygous deletion in mice causes early embryonic lethality, while Rint1 heterozygotes are predisposed to multiple tumor formation. Likewise, rare RINT1 mutations are identified to be associated with intermediate levels of breast cancer risk in human. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-RINT-1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of RINT-1. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected RINT-1 in A431, HeLa, HepG2, and NIH/3T3 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to an N-terminal sequence of human RINT-1. |
| Epitope | N-terminal region. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects RINT-1 by targeting an N-terminal region sequence. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~85 kDa observed. 90.63 kDa calculated. Target band may be resolved into a doublet with the slower migrating band representing product resulting from translation starting at an non-AUG codon (Xiao, J., et Al. (2001). J. Biol. Chem. 276(9):6105-6111). Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in MOLT-4 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected RINT-1 in 10 µg of MOLT-4 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |