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Millipore This Anti-Rint-1 Antibody Is Validated For Use In Western Blotting, Immunocytochemistry For The Detection Of Rint-1. - Mill (Additional S&H or Hazmat Fees May Apply)
RAD50-interacting protein 1 (UniProt Q6NUQ1; also known as HsRINT-1, RINT-1, RAD50 interactor 1) is encoded by the RINT1 gene (Gene ID 60561) in human. Originally identified and named as a RAD50-interacting partner by a yeast two-hybrid screening, RINT1 is involved in the G2/M checkpoint, maintenance of centrosome integrity, telomere elongation, Golgi apparatus, Golgi-ER vesicular trafficking and Cis- and Trans-Golgi homeostasis. Rint1 homozygous deletion in mice causes early embryonic lethality, while Rint1 heterozygotes are predisposed to multiple tumor formation. Likewise, rare RINT1 mutations are identified to be associated with intermediate levels of breast cancer risk in human.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-RINT-1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of RINT-1.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected RINT-1 in A431, HeLa, HepG2, and NIH/3T3 cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to an N-terminal sequence of human RINT-1.
Epitope
N-terminal region.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This polyclonal antibody detects RINT-1 by targeting an N-terminal region sequence.
Species Reactivity
Human
Mouse
Species Reactivity Note
Human, Mouse.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_068749
Gene Symbol
RINT1
Purification Method
Affinity purified.
UniProt Number
Q6NUQ1
Molecular Weight
~85 kDa observed. 90.63 kDa calculated. Target band may be resolved into a doublet with the slower migrating band representing product resulting from translation starting at an non-AUG codon (Xiao, J., et al. (2001). J. Biol. Chem. 276(9):6105-6111). Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in MOLT-4 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected RINT-1 in 10 µg of MOLT-4 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
