Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IH(P), WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABD115 |
| Description | Anti-MUSTN1 |
| Alternate Names |
|
| Background Information | Musculoskeletal embryonic nuclear protein 1 (UniProt: Q8IVN3; also known a MUSTN1) is encoded by the MUSTN1 gene (Gene ID: 389125) in human. MUSTN1 is a small protein of the MUSTANG family that is found predominantly in the musculoskeletal system. It plays a role in the development and regeneration of the musculoskeletal system. It localizes to the nucleus and specifically, spatially in mesenchymal cells of the developing limbs and in the fracture callus in periosteal osteoprogenitor cells, proliferating chondrocytes, and young active osteoblasts. MUSTN1 sequence is highly homologous in vertebrates and contains a nuclear localization sequence without any other significant motifs. MUSTN1 is highly expressed during embryogenesis and is essential for chondrocyte proliferation and differentiation. (Ref.: Gersch, RP and Hadjiargyrou, M (2009). Bone 45(2); 330-338). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH7.4 with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MUSTN1, Cat. No. ABD115, is a rabbit polyclonal antibody that detects human and murine Musculoskeletal embryonic nuclear protein 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2 µg/mL from a representative lot detected MUSTN1 in human heart tissue lysate. Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected MUSTN1 in human skeletal muscle, mouse embryo, and mouse embryonic lung tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Musculoskeletal embryonic nuclear protein 1. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Musculoskeletal embryonic nuclear protein 1 (MUSTN1) in human and mouse. It targets an epitope within 12 amino acids from the C-terminal region. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
|
| Molecular Weight | ~13 kDa observed; 8.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in C2C12 cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected MUSTN1 in C2C12 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |