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The Rex-1 (Zfp-42) gene, which encodes an acidic zinc finger protein, is expressed at high levels in embryonic stem (ES) and F9 teratocarcinoma cells. The Rex-1 promoter is regulated by specific octamer family members in early embryonic cells, and is involved in retinoic acid (RA)-associated reduction in expression. Rex-1 is Involved in self-renewal property of ES cells and may be involved in transcriptional regulation of pluripotency signaling pathways. Rex-1 is also involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency and is required for efficient elongation of TSIX, a non-coding RNA antisense to XIST.
Product Information
Format
Affinity Purified
Control
Undifferentiated H9 human embryonic stem cells.
Presentation
Purified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Rex-1 using this Anti-Rex-1 Antibody validated for use in western blotting & ICC.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Rex-1 in a undifferentiated H9 human embryonic stem cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of Human Rex-1.
Epitope
C-terminus
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_777560
Gene Symbol
REX-1
REX1
ZNF754
Zfp-42
ZFP42
Purification Method
Antigen Affinity Purified
UniProt Number
Q96MM3
Molecular Weight
~35 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in a undifferentiated H9 human embryonic stem cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Rex-1 in undifferentiated H9 human embryonic stem cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
