Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, IH(P), WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1311 |
| Description | Anti-SETD5 |
| Alternate Names |
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| Background Information | SET domain-containing protein 5 (UniProt: Q9C0A6; also known as Setd5) is encoded by the SETD5 (also known as KIAA1757) gene (Gene ID: 55209) in human. Setd5 protein is shown to be essential for mammalian development and is required for regulating germ cell-associated genes in embryonic stem cells. It interacts with Tbl1xr1 and Ctr9, which are components of 2 different transcriptional regulatory complexes (NcoR1 corepressor complex and Paf1 complex) in murine embryonic stem cells. Setd5-deficient embryonic stem cells display impaired cellular proliferation, increased apoptosis, defective cell cycle progression, a diminished ability to differentiate into cardiomyocytes, and greatly perturbed gene expression. Setd5-deficient mouse embryos exhibit severe defects in neural tube formation, somitogenesis and cardiac development, have aberrant vasculogenesis in embryos, yolk sacs, and placentas. They die between embryonic day 10.5 and 11.5. Three isoforms of Setd5 have been reported that are produced by alternative splicing. Its SET domain is localized to amino acids 269-390. Mutation in SETD5 gene has been linked to intellectual disability. (Ref.: Yu, SE., et al. (2017). Cell. Biochem. Funct. 35(5); 247-253; Osipovich, AB., et al. (2016). Development 143(24); 4595-4607). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SETD5, Cat. No. ABE1311, is a highly specific rabbit polyclonal antibody that targets SET domain-containing protein 5 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SETD5 in human cerebral cortex, human pancreas, and human testis tissue sections. Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SETD5 in U2OS cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human SET domain-containing protein 5. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects SET domain containing protein 5 in human cells. It targets an epitope within 19 amino acids from the N-terminal region. |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Rat and Mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~150 kDa observed; 158.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa nuclear extract. Western Blotting Analysis: 0.5 µg/mL of this antibody detected SETD5 in 10 µg of HeLa nuclear extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |