Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1321-25UG |
| Description | Anti-MBD6 |
| Alternate Names |
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| Background Information | Methyl-CpG-binding domain protein 6 (UniProt: Q96DN6; also known as Methyl-CpG-binding protein MBD6) is encoded by the MBD6 (also known as KIAA1887) gene (Gene ID: 114785) in human. The methyl-CpG-binding domain (MBD) proteins are primary candidates for the readout of DNA methylation. MBD proteins require a functional MBD domain and methyl-CpGs, although some MBD proteins can also bind unmethylated DNA. MBD proteins are involved in transcriptional repression by histone modification, nucleosome remodeling or chromatin organization. MBD6 is highly expressed in testes. Its MBD domain is localized in the N-terminal region (aa 11-81) and it also contains proline-rich domains. MBD6 binds to heterochromatin, but does not interact with either methylated or unmethylated DNA. MBD6 is reported to interact directly with the human polycomb deubiquitinase complex, PR-DUB, which catalyzes deubiquitination of H2AK119. Mutations in MBD6 that lead to R883W alteration are reported to cause autism. (Ref.: Cukier, HN., et al. (2012). Autism Res. 5(6); 385-397; Du, Q., et al. (2015). Epigenomics 7(6); 1051-1073). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MBD6, Cat. No. ABE1321, is a highly specific rabbit polyclonal antibody that targets Methyl-CpG-binding domain protein 6 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected MBD6 in HeLa, A431, HEK293, and NIH3T3 cells. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MBD6 in human skin and human stomach tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human Methyl-CpG-binding domain protein 6. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Methyl-CpG-binding domain protein 6 in human and murine cells. It targets an epitope within 16 amino acids from the N-terminal half. |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~110 kDa observed; 101.20 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in SH-SY5Y cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected MBD6 in SH-SY5Y cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |