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Millipore Detect Brg1-Associated Factor 45d Using This Rabbit Polyclonal Anti-Dpf2, Cat. No. Abe1327-25ug, Validated For Use In Western Blotting And Immunocytochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
Zinc finger protein ubi-d4 (UniProt: Q92785; also known as Apoptosis response zinc finger protein, BRG1-associated factor 45D, BAF45D, D4, zinc and double PHD fingers family 2, Protein requiem, DFP2) is encoded by the DPF2 (also known as BAF45D, REQ, UBID4) gene (Gene ID: 5977) in human. BAF45D is a member of the requiem/DPF protein family and is ubiquitously expressed. It may serve as a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. It is also believed to have a role in the development and maturation of lymphoid cells. It contains 3 zinc-finger domains (C2H2-type aa 209-232; PHD type I aa 270-330; and PHD type 2 aa 327-377). Two isoforms of BAF45D have been described that are generated via alternative splicing.
Product Information
Format
Purified
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect BRG1-associated factor 45D using this rabbit polyclonal Anti-DPF2, Cat. No. ABE1327-25UG, validated for use in Western Blotting and Immunocytochemistry.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Western Blotting Analysis: 2 µg/mL from a representative lot detected DPF2 in 10 µg of PC12 cell lysate. Immunocytochemistry Analysis: A 1:100-500 dilution from a representative lot detected DPF2 in A431 and HepG2 cells.
Biological Information
Immunogen
KLH-conjugated liner peptide that corresponding to 17 amino acids from the N-terminal region of human BRG1-associated factor 45D (DPF2).
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects BRG1-associated factor 45D in human, mouse, and rat species. It targets an epitope within 17 amino acids from the N-terminal half. The immunogen sequence is conserved in both isoforms.
Isotype
IgG
Species Reactivity
Human
Rat
Mouse
Species Reactivity Note
Human, Mouse, Rat.
Antibody Type
Polyclonal Antibody
Gene Symbol
DPF2
BAF45D
REQ
UBID4
Purification Method
Affinity Purified
UniProt Number
Q92785
Molecular Weight
44 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in NIH3T3 cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected DPF2 in 10 µg of NIH3T3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
