Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, PIA, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1420-25UG |
| Description | Anti-Mediator complex subunit 25 |
| Alternate Names |
|
| Background Information | Mediator of RNA polymerase II transcription subunit 25 (UniProt: Q71SY5; also known as Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, p78) is encoded by the MED25 (also known as ACID1, ARC92, PTOV2, TCBAP0758) gene (Gene ID: 81857) in human. It is a member of the Mediator complex subunit 25 family that is ubiquitously expressed. Higher expression levels are observed in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen. It acts as a coactivator that is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator complex subunit 25 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Six isoforms of Mediator complex subunit 25 have been described that are generated by alternative splicing. Defects in MED25 gene are known to cause Charcot-Marie-Tooth disease 2B2, a disorder of the peripheral nervous system characterized by progressive weakness and atrophy. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Mediator complex subunit 25, Cat. No. ABE1420-25UG, is a highly specific rabbit polyclonal antibody, that targets MED25 and has been tested in Western Blotting and Peptide Inhibition Assay and Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Mediator complex subunit 25 in human cerebellum and mouse cerebral cortex tissues. Peptide Inhibition Analysis: 2 µg/mL from a representative lot blocked Mediator complex subunit 25 in mouse brain tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 16 amino acids from the internal region of human Mediator of RNA polymerase II transcription subunit 25 (MED25). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Mediator of RNA polymerase II transcription subunit 25 (MED25) in human and murine species. it targets an epitope within 16 amino acids from the internal region. Detects all isoforms of Mediator complex subunit 25. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rat, Yeast, Bovine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
|
| Molecular Weight | 70 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected Mediator complex subunit 25 in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |