Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB, ICC, IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1453-25UL |
| Description | Anti-phospho Brd4 (Ser492/Ser494) |
| Alternate Names |
|
| Background Information | Bromodomain-containing protein 4 (UniProt O60885; also known as MCAP, Mitotic chromosome-associated protein, Protein HUNK1) is encoded by the BRD4 (also known as HUNK1, MCAP) gene (Gene ID 23476) in human. Bromodomain-containing protein 4 is a BET (bromodomain and extra-terminal) protein family member that functions as a chromatin reader by binding acetylated lysines in histones. It plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. It remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for post-mitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, it plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Bromodomain-containing protein 4 is required to form the transcriptionally active P-TEFb complex where it displaces negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb and transforms it into an active form that can then phosphorylate the C-terminal domain of RNA polymerase II. It promotes the phosphorylation of Ser2 of the C-terminal domain of RNA polymerase II. In addition to acetylated histones, Bromodomain-containing protein 4 also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. It is also reported to act as a regulator of p53/TP53-mediated transcription and following phosphorylation by casein kinase 2K2, it is recruited to p53/TP53 specific target promoters. BRD4 knockout in mice is shown to be lethal, while BRD4 function blockage by inhibitor JQ1 (Cat. No. 500586) treatment is reported to affect the transcription of critical synaptic proteins, resulting in memory deficits and decreased seizure susceptibility in mice. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho Brd4 (Ser492/Ser494), Cat. No. ABE1453, is a highly specific rabbit polyclonal antibody that targets Bromodomain-containing protein 4 and has been tested in Dot Blot, Immunocytochemistry, Immunohistochemistry, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected phospho Brd4 (Ser492/Ser494) in whole cell neuronal lysate (C ourtesy of Erica Korb, Ph.D., Rockefeller University) . Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho Brd4 (Ser492/Ser494) in cultured cortical cells from mouse (C47blac6). (C ourtesy of Erica Korb, Ph.D., Rockefeller University) . Dot Blot Analysis: A representative lot detected phospho Brd4 (Ser492/Ser494) in phospho Brd4 peptides (Courtesy of Erica Korb, Ph.D., Rockefeller University). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 14 amino acids surrounding Ser492/Ser494 from the internal N-terminal half of human phospho Brd4 protein. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Bromodomain-conntaining protein 4 phosphorylated at Ser492/Ser494. It targets an epitope of 14 amino acids in the N-terminal half. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Mouse, Rat, and Porcine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
|
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in humans IPS cell lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected phospho Brd44 (Ser492/Ser494) in 10 µg of human IPS cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |