Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R | ChIP, IH(P), WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1851-25UG |
| Description | Anti-MLL-3 |
| Alternate Names |
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| Background Information | Histone-lysine N-methyltransferase 2C (UniProt: Q8BRH4; also known as EC: 2.1.1.43, Lysine-N-methyltransferase 2C, Myeloid-lineage leukemia protein 3 homolog, MLL-3) is encoded by the Kmt2c (also known as Mll3) gene in murine species. Mll3 gene is a member of the myeloid/lymphoid or mixed-lineage leukemia family. MLL-3 protein is a DHHC-type zinc finger protein with six PHD-type zinc fingers, a SET domain, a post-SET domain and a RING-type zinc finger. It methylates Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. MLL-3 is a central component of the MLL2/3 complex, a coactivator complex of nuclear receptors, involved in transcriptional co-activation. In adults, MLL-3 is detected in testis, kidney, spleen and lung and is weakly expressed in brain. It is absent in heart and liver tissue. MLL-3 has been reported to play a role in adipogenesis. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MLL3, Cat. No. ABE1851, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase 2C and has been tested in Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MLL-3 in mouse testis, rat uterus, and mouse kidney tissues. Chromatin Immunoprecipitation Analysis: A representative lot detected MLL-3 in MEF cells (Lee, J., et. Al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8). Western Blotting Analysis: A representative lot detected MLL-3 in Western Blotting applications (Lee, J., et. Al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8); Bres, V., et. Al. (2009). Mol Cell. 36(1):75-87). Immunohistochemistry Analysis: A representative lot detected MLL-3 in mouse urothelial tumors (Lee, J., et. Al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 18 amino acids from the N-terminal region of human MLL-3. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antiody detects MLL-3 protein in human, mouse and rat cells. It targets and epitope within 18 amino acids from the internal region of N-terminal half. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in U2OS nuclear extract. Western Blotting Analysis: 1 µg/mL of this antibody detected MLL-3 in 10 µg of U2OS cell nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |