Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB, ICC, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE1956-25UG |
| Description | Anti-DNMT1 Antibody, AC-4K |
| Alternate Names |
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| Background Information | DNA (cytosine-5)-methyltransferase 1 (UniProt: P26358; also known as EC: 2.1.1.37, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI, MCMT) is encoded by the DNMT1 (also known as AIM, CXXC9, DNMT) gene (Gene ID: 1786) in human. Dnmt1 is an important epigenetic regulator that plays a key role in the maintenance of DNA methylation. Dnmt1 is a homodimeric protein that is ubiquitous in its expression. It is highly expressed in fetal tissues and in heart, kidney, placenta and peripheral mononuclear cells. Its abundance is reduced to almost non-detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Its expression is regulated by many signaling pathways, including PI3/Akt, Rb/E2F and p53/SP1. Dnmt1 is shown to preferentially methylate hemimethylated DNA and it associates with DNA replication sites in the S phase to maintain the methylation pattern in newly synthesized strand, which is essential for epigenetic inheritance. Dnmt1 associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is also responsible for maintaining methylation patterns established in development. Dnmt1 can undergo acetylation on multiple lysines mainly by KAT2B/PCAF. It can be phosphorylated on Serine 154 by Cdks and this phosphorylation is considered to be essential for its enzymatic activity and protein stability. Its phosphorylation on Serine 143 by Akt1 is reported to prevent methylation by SETD7, which increases its stability. Mutations in DNMT1 gene are reported to cause hereditary sensory neuropathy that is characterized by adult onset of progressive peripheral sensory loss associated with progressive hearing impairment and early-onset dementia. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide and 50% glycerol. |
| Applications | |
|---|---|
| Application | Anti-DNMT1, AC-4K, Cat. No. ABE1956, is a highly specific rabbit polyclonal antibody that targets DNA (cytosine-5)-methyltransferase 1 (DNMT1) and has been tested for use in DOT Blot, Immunocytochemistry, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected DNA (cytosine-5)-methyltransferase 1 (DNMT1) in 293T cells transfected with pcmv-Tag2B-DNMT1 (Courtesy of an Independent Researcher). Immunocytochemistry Analysis: A representative lot detected DNA (cytosine-5)-methyltransferase 1 (DNMT1) in Immunocytochemistry applications (Cheng, J., et. Al. (2015). Nat Commun. 6:7023). DOT Blot Analsysi: A representative lot detected DNA (cytosine-5)-methyltransferase 1 (DNMT1) in DOT Blot applications (Cheng, J., et. Al. (2015). Nat Commun. 6:7023). Western Blotting Analysis: A representative lot detected DNA (cytosine-5)-methyltransferase 1 (DNMT1) in Western Blotting applications (Cheng, J., et. Al. (2015). Nat Commun. 6:7023). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 11 amino acids from the C-terminal half with acetylated K1111, 1113, 1115, and 1117. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects DNA (cytosine-5)-methyltransferase 1 acetylated on four lysine residues from the C-terminal half containing six tandem repeats of K-g. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Mouse and Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~200 kDa observed; 183.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by DOT Blot in DNMT1-Control peptide & DNMT1-4Ac peptide. DOT Blot Analysis: A 1:500 dilution of this antibody detected DNA (cytosine-5)-methyltransferase 1 with acetylated Lysine 1111, 1113, 1115, and 1117. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |