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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ChIP | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE212 |
| Description | Anti-Set1A Antibody |
| Alternate Names |
|
| Background Information | Set1A is a histone methyltransferase (HMT) that is part of the SET1 HMT complex. This protein specifically methylates lysine 4 of histone H3 if the adjacent lysine at position 9 is also unmethylated. Studies demonstrate that Set1A and Set1B localize to largely non-overlapping regions of the chromosome. This suggests Set1A and Set1B bind to unique sets of target genes and that each protien makes non-redundant contributions to the epigenetic control of chromatin structure and gene expression. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal serum containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Set1A Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Set1A also known as SET domain containing 1A, Set1/Ash2 histone methyltransferase complex subunit SET1. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP. See Lee, J.H., et Al.(2008). Mol Cell Biol. 28(2):609–618. Immunocytochemistry: A representative lot of this antibody was reported to work in immunocytochemistry. See . Lee, J.H., et Al. (2007). J Biol Chem. 282(18):13419-13428. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human Set1A. |
| Host | Rabbit |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Histone methyltransferase that specifically methylates Lys-4 of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring Lys-9 residue is already methylated. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. CATALYTIC ACTIVITY: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]. SUBUNIT STRUCTURE: Interacts with HCFC1. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2/ASH2L and CXXC1/CFP1. SUBCELLULAR LOCATION: Nucleus speckle. Chromosome. Note: Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes. SEQUENCE SIMILARITIES: Contains 1 post-SET domain. Contains 1 RRM (RNA recognition motif) domain. Contains 1 SET domain. SEQUENCE CAUTION: The sequence AAH27450.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH35795.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence BAA20797.2 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | ~186 kDa observed. A cross-reacting band appears at ~45 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in PC3 cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected Set1A on 10 µg of PC3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |