The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies.Learn about our cookie policy.
Millipore This Anti-Phospho Histone H3 (Ser57) Antibody, Trial Size Is Validated For Use In Western Blotting, Icc, Ip & DOT Blot For The Detection Of Phospho Histone H3 (Ser57). - Mill (Additional S&H or Hazmat Fees May Apply)
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-phospho Histone H3 (Ser57) Antibody, Trial Size is validated for use in western blotting, ICC, IP & DOT blot for the detection of phospho Histone H3 (Ser57).
Key Applications
Western Blotting
Immunocytochemistry
Immunoprecipitation
DOT Blot
Application Notes
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho Histone H3 (Ser57) in HeLa cells.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho Histone H3 (Ser57) from 500 µg of colcemid treated HeLa acid extract.
DOT Blot (Specificity) Analysis: 0.005 µg/mL from a representative lot detected phospho Histone H3 (Ser57) in a panel containing various modified and non-modified Histone H3 peptides.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Ser57.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Human
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_003520
Gene Symbol
HIST1H3A
H3FA
HIST1H3B
H3FL
HIST1H3C
H3FC
HIST1H3D
H3FB
HIST1H3E
H3FD
HIST1H3F
H3FI
HIST1H3G
H3FH
HIST1H3H
H3FK
HIST1H3I
H3FF
HIST1H3J
H3FJ
Modifications
Phosphorylation
Purification Method
Affinity Purfied
UniProt Number
P68431
Molecular Weight
~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in colcemid treated HeLa acid extract.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho Histone H3 (Ser57) in 10 µg of colcemid treated HeLa acid extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
