Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IH(P), WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE2586 |
| Description | Anti-NR2F2 (ARP-1) isoform B |
| Alternate Names |
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| Background Information | COUP transcription factor 2 (UniProt: P24468-2; also known as COUP-TF2, COUP-TF II, Apolipoprotein A-I regulatory protein 1, ARP-1, COUP transcription factor II, COUP-TF II, Nuclear receptor subfamily 2 group F member 2) is encoded by the NR2F2 (also known as ARP1, TFCOUP2) gene (Gene ID: 7026) in human. Coup-TF II is a ligand-activated transcription factor that is activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid. Its DNA binding region is localized in amino acids 76-151. COUP-TF II contains two NR C4-type zinc finger domains (aa 79-99 and 115-139). It is widely distributed in human tissues with detectable expression in all tissues examined. Its tissue and cell-specific expression can be modulated both transcriptionally and post-translationally. Through binding to 5 -AGGTCA-3 direct repeats (DRs) with variable spacing, it can modulate the expression of target genes, such as RA receptor beta 2, PEPCK, NGFI-A, and cholesterol 7 -hydroxylase. COUP-TF II action can also be enhanced by interaction with certain coactivators, such as with members of the steroid receptor coactivator family members SRC-1, SRC-2, and SRC-3. Mutations in NR2F2 gene have been linked to multiple types of congenital heart defects characterized transposition of the great arteries, aortic stenosis, and ventricular septal defect. (Ref.: Litchfield, LM and Klinge, CM (2012). J. Mol. Endocrinol. 49 (3); R135-R148). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NR2F2 isoform B, Cat. No. ABE2586, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 2 group F member 2. and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2 µg/mL from a representative lot detected NR2F2 isoform B in in 10 µg of L6 cell lysate. Western Blotting Analysis: A representative lot detected NR2F2 isoform B in MEWO cells overexpressing NR2F2 isoform B.(Courtesy of Claudia Lovell at Hernando Lab, NYU Langone Medical Center). Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected NR2F2 isoform B in human kidney, human pancreas, and human cerebellum tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the first 14 amino acids from the N-terminus region of human COUP transcription factor 2 (ARP1), isoform B. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects COUP-TF II. It targets an epitope within 14 amino acids from the N-terminus region of isoform B. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Rat. Predicted to react with Human, Sheep, Mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~37kDa observed; 31.48 kDa calculated for isoform B. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in PC12 cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected NR2F2 isoform B in in 10 µg of PC12 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |