Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | ICC, IF, IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE2619 |
| Description | Anti-Polo-like kinase-1 |
| Alternate Names |
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| Background Information | Serine/threonine-protein kinase PLK1 (UniProt: P53350; also known as EC: 2.7.11.21, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13) is encoded by the PLK1 (also known as PLK) gene (Gene ID: 5347) in human. PLK-1 is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. It is shown to express in placenta and colon and its activity is enhanced by growth promoting agents. PLK-1 is activated by phosphorylation at Thr210 by aurora kinase A. It accumulates to a maximum during the G2 and M phases and declines to a nearly undetectable levels following mitosis and throughout G1 phase. However, it begins to accumulate again during the S phase of cell cycle. The kinase domain of PLK-1 is localized to amino acids 53-305 with activation loop in the region of amino acids 194-221. PLK-1 contains 2 POLO box domains (aa 417-480 and 515-584), which act as phosphopeptide-binding module that recognize and bind serine-phosphothreonine/ phosphoserine]-(proline/X) motifs. PLK-1 recognizes and binds docking proteins that are already phosphorylated on these motifs, and then phosphorylates them. Defects in PLK1 gene are associated with some cancers, such as gastric, thyroid or B-cell lymphomas. Expression is shown to be higher in tumor tissues with a poor prognosis, suggesting its role in malignant transformations and carcinogenesis. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS containing 80 mM Glycine with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Polo-like kinase-1, Cat. No. ABE2619, is a rabbit polyclonal antibody that detects Polo-Like Kinse 1 and has been tested for use Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A representative lot detected Polo-like kinase-1 in U2OS cells (Macurek, L., et. al. (2008). Nature. 455(7209):119-23). Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Polo-like kinase-1 in U2OS and C2C12 cells. Western Blotting Analysis: A representative lot detected Polo-like kinase-1 in U2OS-TR transfected cells (Macurek, L., et. al. (2008). Nature. 455(7209):119-23). Immunoprecipitation Analysis: A representative lot detected Polo-like kinase-1 in U2OS-TR transfected cells (Macurek, L., et. al. (2008). Nature. 455(7209):119-23). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant fragment corresponding to 303 amino acids from the C-terminal half of human Polo-like kinase-1. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects human Polo-like kinase 1. It targets an epitope within 303 amino acids from the C-terminal half. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~68 kDa observed; 68.26 kD calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in U2OS transfected with PLK1 siRNA and U2OS transfected with control oligo (LUC o Luciferase). Western Blotting Analysis: 0.5 µg/mL of this antibody detected Polo-like kinase-1 in U2OS transfected with PLK1 siRNA (PLK1) & U2OS transfected with control oligo (LUC o Luciferase). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |