Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE2626 |
| Description | Anti-PASD1 |
| Alternate Names |
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| Background Information | Circadian clock protein PASD1 (UniProt: Q8IV76; also known as Cancer/testis antigen 63, CT63, OC-TES 11, PAS domain-containing protein 1) is encoded by the PASD1 gene (Gene ID: 139135) in human. PASD1 is evolutionarily nuclear protein that associates preferentially at the periphery of the nucleus with heterochromatin. Human PASD1 contains an N-terminal PAS domain (a.a. 30-102), a central region (a.a. 365-412) essential for its transcriptional repression activity, and two coiled coil domains (CC1/a.a. 365-412 and CC2/a.a. 475-553), where CC1 exhibits significant homology with CLOCK exon 19-coded sequence. PASD1 is related to CLOCK and interacts with the bHLH-PAS transcription complex CLOCK-BMAL1 to repress transcriptional activation. PASD1 expression is normally limited to immunologically restricted germline tissues that do not express class I MHC molecules, such as testis and placenta, allowing tissue-specific circadian rhythms suppression. It is also expressed in a broad range of cancer cells, including melanoma, lung cancer, and breast cancer. It displays widespread expression in solid tumors and diffuse large B-cell lymphoma (DLBCL)-derived cell lines. Isoform 2 is expressed in all DLBCL-derived cell lines, while isoform 1 is preferentially expressed in cell lines derived from non-germinal center DLBCL. When over-expressed, PASD1 inhibits circadian clock function in cancer cells and reducing PASD1 in cancer cells significantly increases the amplitude of transcriptional oscillations to generate more robust circadian rhythms. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PASD1, Cat. No. ABE2626, is a highly specific rabbit polyclonal antibody that targets Circadian clock protein PASD1 and has been tested in Immunoprecipitation and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected PASD1 in H1299 cells (Michael, A.K., et. Al. (2015). Mol Cell. 58(5):743-54). Immunoprecipitation Analysis: 5 ug from a representative lot immunoprecipitated PASD1 in 500 µg of SW480 lysate. Western Blotting Analysis: A representative lot detected PASD1 in HEK293T transfected myc-his Tagged PASD1 lysate V2 isoform (Courtesy of Dr Carrie Partch at University of California Santa Cruz). Immunoprecipitation Analysis: A representative lot detected PASD1 in H1299 & SW480 cells (Courtesy of Dr Carrie Partch at University of California Santa Cruz). Immunoprecipitation Analysis: A representative lot detected PASD1 in H1299 and SW480 cell lysates (Michael, A.K., et. Al. (2015). Mol Cell. 58(5):743-54). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 19 amino acids from the C-terminal end of human PASD1, isoform 1. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects PASD1, isoform 1 version 2, in human cells. It targets an epitope within 19 amino acids from the C-terminal end. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~120 kDa observed; 87.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293T cells transfected myc-His Tagged PASD1 lysate V2 isoform. Western Blotting Analysis: 0.2 µg/mL of this antibody detected PASD1 in lysate from HEK293T cells transfected myc-His Tagged PASD1, isoform 1, version2. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |