Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ChIP, IF, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE2865-100UG |
| Description | Anti-Meis1/2 |
| Alternate Names |
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| Background Information | Homeobox protein Meis1 (UniProt: Q60954-1; also known as Myeloid ecotropic viral integration site 1) Homeobox protein Meis2 (UniProt: P97367-2; also known as Meis1-related protein 1) are encoded by the Meis1 and Meis2 (also known as Mrg1, Stra10) genes (Gene ID: 17268 and 17536) in murine species. Meis proteins act as transcription factors that are orthologous to the Drosophila homothorax (Hth) protein. They are expressed dynamically in several organs during embryogenesis and exert potent regulatory activity through their interaction with Hox proteins and other transcription factors. Meis protein contain a TALE (three-amino-acid loop extension) sub-class of the homeodomain that binds to DNA. They directly bind to Pbx proteins and the Meis/Pbx protein complex binds to DNA through respective Meis- and Pbx-consensus binding sites to regulate transcription. The Meis/Pbx complex plays important roles during development of multiple organs. In humans and mice, three homologues Meis1, Meis2 and Meis3 have been identified. Meis1 is expressed at high levels in the lung and at lower levels in the heart and brain. On the other hand, Meis 2 displays spatially restricted expression patterns in the developing nervous system, limbs, face, and in various viscera. In adult, it is mainly expressed in the brain and female genital tract, with a different distribution of the alternative splice forms in these organs. Pancreatic beta cells express both Meis1 and 2. Both Meis1 and 2 are expressed at high levels in all stages of embryonic development of mice (day 7 to 17). Meis3 is expressed in pancreatic islets and is reported to control the expression of expression of PDK1, which promotes survival of pancreatic beta-cells. Deficiency of Meis1 or Meis 2 can lead to embryonic lethality. Meis1-deficient mice die around midgestation due to a general failure of definitive hematopoiesis. (Ref.: Machon, O et Al. (2015). BMC Dev. Biol. 515:40; Azcoitia, V et Al., (2005). Dev. Biol. 280 (2); 307-320). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol. |
| Applications | |
|---|---|
| Application | Anti-Meis1/2 Antibody, Cat. No. ABE2865, is a highly specific rabbit polyclonal antibody that targets Meis1/2 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunofluorescence, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A representative lot detected Meis1/2 in Immunofluorescence applications (Mercader, N., et. Al. (2005). Development. 132(18):4131-42; Marcos, S., et. Al. (2015). Development. 142(17):3009-20). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Meis1/2 in Chromatin Immunoprecipitation applications (Penkov, D., et. Al. (2013). Cell Rep. 3(4):1321-33; Marcos, S., et. Al. (2015). Development. 142(17):3009-20). Western Blotting Analysis: A representative lot detected Meis1/2 in Western Blotting applications (Mercader, N., et. Al. (2005). Development. 132(18):4131-42). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of murine Meis1a, Meis2a, and 13 amino acids from Meis2b isoforms. |
| Epitope | C-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Meis1/2. It targets an epitope with in 14 amino acids from the C-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Predicted to react with Salamander, Human based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A purified |
| UniProt Number |
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| Molecular Weight | ~51 kDa observed; 43.00, 43.79, and 43.06 kDa for Meis1a, Meis2a, and Meis2b, respectively. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293T cells transfected with pCMVTAG2-Flag-Meis2a. Western Blotting Analysis: 2 µg/mL of this antibody detected Meis1/2 in HEK293T cells transfected with pCMVTAG2-Flag-Meis2a. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |