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Millipore Anti-Orf0 Antibody, Cat. No. Abe2895, Is A Highly Specific Rabbit Polyclonal Antibody That Targets Orf0 And Has Been Tested In Immunocytochemistry, Immunoprecipitation, And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Open Reading Frame 0 (ORF0) is a primate-specific open reading frame in LINE-1 retrotransposons. Over 3,000 potential ORF0 loci are reported to be present in human and chimpanzee genomes. ORF0 is a predominantly nuclear protein that enhances LINE-1 mobility and contributes to retrotransposon-mediated diversity. It is a highly positively charged protein that may act by binding to nucleic acids. It displays two splice donor (SD) sites at nucleotides 106 and 191 (amino acids 35 and 64) that can act in concert with splice acceptors (SAs) in downstream genomic sequences to generate fusion proteins. ORF0 transcripts are reported to exist in induced pluripotent stem cells (iPSc) derived from primates. (Ref.: Denli, AM et al (2015). Cell 163(3): 583 593).
Product Information
Format
Unpurified
Presentation
Rabbit polyclonal antiserum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-ORF0 Antibody, Cat. No. ABE2895, is a highly specific rabbit polyclonal antibody that targets ORF0 and has been tested in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
Immunocytochemistry
Immunoprecipitation
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).
Immunoprecipitation Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).
Immunocytochemistry Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).
Biological Information
Immunogen
KLH-conjugated linear peptides corresponding to ORF0 amino acid residues 20-34, 33-49, and 50-65.
Epitope
unknown
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody recognizes primate-specific open reading frame 0 (ORF0).
Isotype
IgG
Species Reactivity
Primate
Species Reactivity Note
Primate. Predicted to react with Chimpanzee and Human based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Gene Symbol
ORF0
Purification Method
Unpurified
UniProt Number
N/A
Molecular Weight
~16 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in extracts from HEK293T cells transfected with ORF0 expression plasmid.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected ORF0 in lysate from HEK293T cells transfected with ORF0 expression plasmid.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
