Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Pm | IP, ICC, WB | Rb | Unpurified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE2895-25UL |
| Description | Anti-ORF0 |
| Alternate Names |
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| Background Information | Open Reading Frame 0 (ORF0) is a primate-specific open reading frame in LINE-1 retrotransposons. Over 3,000 potential ORF0 loci are reported to be present in human and chimpanzee genomes. ORF0 is a predominantly nuclear protein that enhances LINE-1 mobility and contributes to retrotransposon-mediated diversity. It is a highly positively charged protein that may act by binding to nucleic acids. It displays two splice donor (SD) sites at nucleotides 106 and 191 (amino acids 35 and 64) that can act in concert with splice acceptors (SAs) in downstream genomic sequences to generate fusion proteins. ORF0 transcripts are reported to exist in induced pluripotent stem cells (iPSc) derived from primates. (Ref.: Denli, AM et al (2015). Cell 163(3): 583 593). |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-ORF0 Antibody, Cat. No. ABE2895, is a highly specific rabbit polyclonal antibody, that targets and has been tested in Immunoprecipitation, Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93). Immunoprecipitation Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93). Immunocytochemistry Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptides corresponding to ORF0 amino acid residues 20-34, 33-49, and 50-65. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody recognizes primate-specific open reading frame 0 (ORF0). |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Primate. Predicted to react with Chimpanzee and Human based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | 16 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in extracts from HEK293T cells transfected with ORF0 expression plasmid. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected ORF0 in lysate from HEK293T cells transfected with ORF0 expression plasmid. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |