Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB, IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE2897-100UG |
| Description | Anti-phospho GATA6 (Ser37) |
| Alternate Names |
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| Background Information | Transcription factor GATA-6 (UniProt: Q92908; also known as GATA-binding factor 6) is encoded by the GATA6 gene (Gene ID: 2627) in human. GATA6 is a zinc-finger transcription factor that belongs to the six-member family of transcription factors that bind to the consensus sequence (A/T)GATA(A/g). GATA1, GATA2, and GATA3 are mainly expressed in hematopoietic cell lineages, while GATA4, GATA5, and GATA6 are predominantly expressed in the heart, gonads, and endodermal-derived tissues. It has two zinc-finger repeats localized to amino acids 390-414 and 444-468, which mediate interaction with LIM and cysteine-rich domains protein 1 (LMCD1). GATA-6 is shown to be important in the endodermal differentiation of organ tissues and has been indicated in proper lung development by controlling the late differentiation stages of alveolar epithelium and aquaporin-5 promoter activation. It has also been linked to the production of LIF that encourages proliferation of endodermal embryonic stem cells and blocks early epiblast differentiation. GATA6 is also required for definitive endoderm specification in human embryonic stem cells and induced pluripotent stem cells. Phosphorylation of GATA6 by GSK-3 on serine 37 is reported to promote its degradation, thereby preventing GATA6 from repressing transcripts that are induced by TNF and attenuated by insulin. Two isoforms of GATA-6 have been reported that are produced by alternative initiation. (Ref.: Chitforoushzadeh, Z., et Al. (2016). ScienceSignaling 9 (431), ra59; Tiyaboonchai, A., et Al. (2017). Stem Cell Reports 8 (3); 589-604). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-phospho GATA6 (Ser37), Cat. No. ABE2897, is a highly specific rabbit polyclonal antibody that targets Transcription factor GATA-6 (GATA-binding factor 6) and has been tested for use in DOT Blot, Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected phospho GATA6 (Ser37) in 293T cells transfected with 3XFLAG-tagged GATA6L. Western Blotting Analysis: A representative lot detected phospho GATA6 (Ser37) in Western Blotting applications (Chitforoushzadeh, Z., et. Al. (2016). Sci Signal. 9(431):ra59). Immunoprecipitation Analysis: 4 µg/mL from a representative lot immunoprecipitated phospho GATA6 (Ser37) in 293T cells were transfected with 3xFLAG-GATA6 (Courtesy of Dr. Kevin A. Janes at University of Virginia). Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho GATA6 (Ser37) in Immunoprecipitation applications (Chitforoushzadeh, Z., et. Al. (2016). Sci Signal. 9(431):ra59). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphoserine 37 of human Transcription factor GATA-6. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects GATA6 phosphorylated on Serine 37. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Rat, Mouse, Porcine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~75 kDa observed; 60.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | DOT Blot Analysis: A 1:500 dilution from a representative lot detected phospho GATA6 (Ser37) in Phosphorylated GATA6 peptide. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |