| Description | |
|---|---|
| Catalogue Number | ABE2909-25UL |
| Description | Anti-phospho Histone H4 (Tyr51) |
| Alternate Names |
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| Background Information | Histone H4 (UniProt: P62805 ) is encoded by the HIST14A (also known as H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/g, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4) gene (Gene ID: 121504, 554313, 8294, 8359, 8360, 8361, 8362, 8363, 8364, 8365, 8366, 8367, 8368, 8370) in human. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 is one of the main histone proteins that contribute to the chromatin structure in both prokaryotic and eukaryotic cells. Histone H4 can undergo several different types of epigenetic modifications that influence cellular processes, including methylation, acetylation, and phosphorylation. Acetylation at Lys6, Lys9, Lys13 and Lys17 is shown to occur in coding regions of the genome but not in heterochromatin. Monomethylation and asymmetric dimethylation at Arg4 is reported to favor acetylation at Lys9 and Lys13. Histone H4 can be phosphorylated by Tie2 on Tyr51, which is shown to preferentially occur in the presence of core histones. The phosphorylated H4 (H4Y51) is recognized by the proto-oncogene ABL1. Histone H4 is also phosphorylated on Ser48 by PAK2, which is shown to increase the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Chromosomal aberrations involving histone H4 causes of B-cell non-Hodgkin lymphomas. (Ref.: Hossain, MB., et Al. (2016). Sci. Adv. 2; e1501290). |
| Product Information | |
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| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody containing PBS without preservatives and 40% glycerol. |
| Applications | |
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| Application | Anti-phospho Histone H4 (Tyr51) Antibody, Cat. No. ABE2909, is a highly specific rabbit polyclonal antibody that targets Histone H4 phosphorylated on Tyr51 and has been tested for use in in Immunocytochemistry, Immunofluorescence, and Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A representative lot detected Tyr51 phosphorylation of Histone H4 in the presence of active recombinant Tie2, following analysis by Western blotting. (Hossain, MB., et Al. (2016). Sci. Adv. 2; e1501290). Immunofluorescence Analysis: A representative lot detected phospho Histone H4 (Tyr51) in HEK293 Tie2 cells treated with Angiopoietin 1 ( ANG1) and ionizing radiation (Hossain, MB., et Al. (2016). Sci. Adv. 2; e1501290). |
| Biological Information | |
|---|---|
| Immunogen | Two linear peptides (phosphorylated on Tyr 51 and non-phosphorylated) corresponding to 11 amino acids from the internal region of human Histone H4. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Histone H4 phosphorylated on Tyrosine 51. It targets an epitope within 11 amino acids surrounding phosphotyrosine 51. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | 11.38 kDa calculated. |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Immunocytochemistry in HEK293 Tie2-myc cells treated with Angiopoietin 1 ( ANG1). Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected phospho Histone H4 (Tyr51) in HEK293 Tie2-myc cells treated with Angiopoietin 1 ( ANG1) . |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at -20°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 µL |