Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IF, WB, ICC | Rb | Unpurified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE2910-25UL |
| Description | Anti-DNMT3L |
| Alternate Names |
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| Background Information | DNA (cytosine-5)-methyltransferase 3-like (UniProt: Q9CWR8; also known as DNMT3L) is encoded by the Dnmt3l gene (Gene ID: 54427) in murine species. DNMT3L is a catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context. It is shown to be essential for the function of DNMT3A and DNMT3B. It activates DNMT3A and DNMT3B by binding to their catalytic domain. It acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. DNMT3L recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. In germ cells, it is required for the methylation of imprinted loci together with DNMT3A. In male germ it is essential for the methylation of retrotransposons, which prevents their mobilization. DNMT3A also plays a key role in embryonic stem cells (ESCs) by acting both as a positive and negative regulator of DNA methylation. It promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B. It can also serve as an inhibitor of DNA methylation at the promoter of bivalent genes. It is reported to promote differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression. In testicular cells it is first observed in non-dividing prospermatogonia after 12.5 dpc and its level reaches its highest at about the time of birth and then rapidly declines and is non-detectable six days postpartum when most of the prospermatognia are differentiated into dividing spermatogonial stem cells. |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-DNMT3L, Cat. No. ABE2910, is a highly specific rabbit polyclonal antibody, that targets DNA (cytosine-5)-methyltransferase 3-like and has been tested in Immunofluorescence, Western Blotting and Immunocytochemistry. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Dnmt3l in undifferentiated J1 mouse embryonic stem cells (Nimura, K., et. al. (2006). Genes Cells. 11(10):1225-37). Immunofluorescence Analysis: A representative lot detected Dnmt3l in mouse testes (Nimura, K., et. al. (2006). Genes Cells. 11(10):1225-37) and XY germ cells at E15.5 (Kato, Y., et. al. (2016). Nat Commun. 7:11272). Immunocytochemistry Analysis: A representative lot detected Dnmt3l in undifferentiated J1 mouse embryonic stem cells (Nimura, K., et. al. (2006). Genes Cells. 11(10):1225-37). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full length (421 aa) murine recombinant DNA (cytosine-5)-methyltransferase 3-like (DNMT3L). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Dnmt3l in murine cells. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | 50 kDa observed. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse embryonic stem cell lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Dnmt3l in 10 µg of mouse embryonic stem cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |