Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB, PIA, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE344-25UG |
| Description | Anti-Acetyl MLL1 (KMT2A) (Lys 1130/1133) |
| Alternate Names |
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| Background Information | Histone-lysine N-methyltransferase 2A (UniProt: Q03164; also known as EC: 2.1.1.43, Lysine N-methyltransferase 2A, ALL-1, CXXC-type zinc finger protein 7, Myeloid/lymphoid or mixed-lineage leukemia, Myeloid/lymphoid or mixed-lineage leukemia protein 1, MLL1, Trithorax-like protein, Zinc finger protein HRX) is encoded by the KMT2A (also known as ALL1, CXXC7, HRX, HTRX, MLL, MLL1, TRX1) gene (Gene ID: 4297) in human. It is expressed in heart, lung, and brain and in T- and B-lymphocytes and plays an essential role in early development and hematopoiesis. Proteolytic cleavage of MLL1 by Taspase 1 (TASP1) generates an N-terminal cleavage product of 320 kDa and a C-terminal product of 180 kDa. The catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of Lysine 4 of histone H3 (H3K4me) complex and acetylation of Lysine 16 of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. MLL1 displays weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. It does not exhibit any activity toward histone H3 phosphorylated on Threonine 3, and less activity toward H3 dimethylated on Arginine 8 or Lysine 9. However, it has higher activity toward H3 acetylated on Lysine 9. MLL1 is shown to be required for transcriptional activation of HOXA9 and it promotes PPP1R15A-induced apoptosis. MLL1 is reported to coordinate recruitment of CLOCK BMAL1 activator complexes to chromatin, an event associated with cyclic trimethylation of histone H3 Lysine 4 (H3K4) at circadian promoters. MLL1 is also reported to play a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-ARNTL/BMAL1 heterodimer. (Ref.: Aguilar-Arnal, L., et Al. (2015). Nat. Struct. Mol. Biol. 22(4); 312-318). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-GLYCINE (pH 7.4), 150mM NaCl, with 0.05% Sodium Azide without glycerol. |
| Applications | |
|---|---|
| Application | Anti-Acetyl MLL1 (KMT2A) (Lys 1130/1133), Cat. No. ABE344, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase 2A (MLL1) acetylated on Lysine 1130/1133 and has been tested for use in DOT Blot, Peptide Inhibition Assay, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Acetyl MLL1 (KMT2A) (Lys 1130/1133) in Western Blotting applications (Aquilar-Arnal, L., et. Al. (2015). Nat Struct Mol Biol. 22(4):312-8). Peptide Inhibition Analysis: A representative lot was used with Acetylated peptide (Ag peptide) and Control peptide for peptide block analysis. (Courtesy of Dr. Lorena Aguilar-Arnal) |
| Biological Information | |
|---|---|
| Immunogen | KLH-cpnugated linear peptide corresponding to 12 amino acids surrounding acetylated lysines 1130 and 1133 from the N-terminal half of human Histone-lysine N-methyltransferase 2A. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects human Histone-lysine N-methyltransferase 2A acetylated on lysine 1130 and 1133. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | 431.76 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by DOT Blot with Acetylated peptide (Ag peptide) and Control peptide. DOT Blot Analysis: 1 µg/mL of this antibody detected Acetyl MLL1 (KMT2A) peptide acetylated on Lysine 1130/1133, but did not dectect the control peptide. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |