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Millipore This Anti-Pabp-2 Antibody Is Validated For Use In Western Blotting, Ip & Icc For The Detection Of Pabp-2. - Mill (Additional S&H or Hazmat Fees May Apply)
PABPN1, also known as Polyadenylate-binding protein 2, Poly(A)-binding protein II (PABP-2), Poly(A)-binding protein 2, PABII, Nuclear poly(A)-binding protein 1,or Polyadenylate-binding nuclear protein 1, and encoded by the gene PABPN1/PAB2/PABP2, is a RNA binding protein involved in the 3-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tails. PABPN1 stimulates poly(A) polymerase (PAPOLA) tail elongation and controls the poly(A) tail length. PABPN1is also important in nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA and appears to also protect the poly(A) tail from degradation. PABPN1 is localized to the nucleus and cytoplasm. PABPN1 is found in cytoplasmic mRNP granules containing untranslated mRNAs and shuttles between the nucleus and the cytoplasm but is predominantly found in the nucleus.
Product Information
Format
Serum
Presentation
Rabbit polyclonal in buffer containing serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-PABP-2 antibody is validated for use in western blotting, IP & ICC for the detection of PABP-2.
Key Applications
Western Blotting
Immunoprecipitation
Immunocytochemistry
Application Notes
Immunoprecipitation Analysis: A representative lot immunoprecipitated PABP-2 in mouse limb tissue lysate. Immunocytochemistry Analysis: A representative lot detected PABP-2 in mouse limb cells.
Biological Information
Immunogen
Recombinant protein corresponding to bovine PABP-2.
Host
Rabbit
Species Reactivity
Human
Rat
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_776994
Gene Symbol
PABPN1
PAB2
PABP2
Purification Method
Unpurified
UniProt Number
Q28165
Molecular Weight
~ 50 kDa observed. The calculated molecular weight is 34 kDa, however PABP-2 has been shown as a ~50 kDa band in western blots (Nemeth A. K, et Al. (1995). Nucleic Acids Res. 23(20):4034-41).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PABP-2 in 10 µg of A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
