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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE415 |
| Description | Anti-Transcription factor EB Antibody |
| Alternate Names |
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| Background Information | The protein named Transcription factor EB (TFEB) or alternatively in older literature, Class E basic helix-loop-helix protein 35 (bHLHe35) and encoded by the human gene TFEB/BHLH35 is a transcription factor that specifically recognizes and binds E-box sequences (5-CANNTG-3) on DNA. Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. TFEB in association with the protein TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB also plays a central role in expression of lysosomal genes. TFEB specifically recognizes and binds the CLEAR-box sequence (5-GTCACGTGAC-3) present in the regulatory region of many lysosomal genes and through binding acts as a positive regulator of their expression. Finally, TFEB appears to be a positive regulator for autophagy related gene expression, heavy chain immunoglobulin gene expression, and genes necessary for the proper vascularization of important tissues such as the placenta. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect TFEB using this rabbit polyclonal antibody, Anti-Transcription factor EB Antibody validated for use in western blotting & IP. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Transcription factor EB in 500 µg of Raji cell lysate. Immunopecipitated Transcription factor EB was then detected in Western Blot. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a region near the C-terminus of human Transcription factor EB. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Rhesus Macaque, Gibbon, Orangutan, Baboon, and Chimpanzee based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~58 kDa and ~40 kDa observed. Uniprot describes two isoforms at 44 kDa and 53 kDa. This protein has been observed at 44-53 kDa due to sumoylation (Miller, A. J., et Al. (2005). J Biol Chem. 280(1):146-55.). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Raji cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Transcription factor EB in 10 µg of Raji cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |