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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE477 |
| Description | Anti-BRD2 Antibody |
| Alternate Names |
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| Background Information | Bromodomain-containing protein 2 (BRD2) is a member of the BET subfamily of bromodomain proteins. These proteins contain two tandem N-terminal bromodomains and a single C-terminal extra-terminal domain. Studies have shown BRD2 to be associated with several regulators of transcription (eg. TFIID and Swi/Snf complexes) in addition to its involvement in guiding cell cycle gene expression through its binding to multiple E2F. BRD2 is hypothesized to function in mammalian development by regulating chromatin structure and transcription. Consistent with this hypothesis, studies have shown BRD2 to bind histone H4 via acetylated Lys12. BRD2 mutants have been associated with increased susceptibility to juvenile myoclonic epilepsy. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-BRD2 Antibody is a highly specific rabbit polyclonal antibody, that targets BRD2 & has been tested in western blotting & IHC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:500 dilution of this antibody from a representative lot detected BRD2 in human testis tissues. Immunohistochemistry Analysis: A 1:2,000 and a 1:500 dilution from a representative lot detected BRD2 in rat testis tissues. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to a region near the C-terminus of human BRD2. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody is expected to cross react with isoforms 1 and 2 of BRD2, based upon sequence homology. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~110 kDa observed. Uniprot describes two isoforms at 88 kDa (isoform 1) and 92 kDa (isoform 2). Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 0.25 µg/mL of this antibody detected BRD2 in 10 µg of Jurkat cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |