Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE484 |
| Description | Anti-LOXL2 |
| Alternate Names |
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| Background Information | Lysyl oxidase homolog 2 (UniProt: Q9Y4K0; also known as EC: 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14, LOXL2) is encoded by the LOXL2 gene (Gene ID: 4017) in human. LOXL2 localizes to both cytoplasmic and nuclear region. It catalyzes the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, it promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOXL2 also acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When present in the nucleus, it acts as a transcription corepressor and specifically mediates deamination of trimethylated Lysine 4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Overexpression of LOXL2 has been reported in a number of cancers and its ability to promote epithelial to mesenchymal transition suggest that it might play a role in tumor progression. Higher expression has been correlated with metastasis and reduced survival in patients with aggressive breast cancer. LOXL2 activity is strongly induced in hypoxia and it is reported to be a direct transcriptional target of HIF1A. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-LOXL2, Cat. No. ABE484, is a highly specific rabbit polyclonal antibody that targets Lysyl oxidase homolog 2 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:500 dilution from a representative lot detected LOXL2 in MDA-MB-231 cell lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LOXL2 in human prostate and human placenta tissues. Immunoprecipitation Analysis: A representative lot detected LOXL2 in LoxL2 expressing vector (Courtesy Dr Sandra Peiro from the Institut Hospital del Mar dInvestigacions Mèdiques (IMIM) in Barcelona Spain). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human Lysyl oxidase homolog 2. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Lysyl oxidase homolog 2 in human cells. It targets an epitope within 16 amino acids from the N-terminal half. |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Bovine, Mouse, Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~82 kDa obseerved; 86.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected LOXL2 in A431 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |