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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE496 |
| Description | Anti-Lysine-specific demethylase 5C Antibody |
| Alternate Names |
|
| Background Information | Lysine-specific demethylase 5C is also called Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX and Protein Xe169. Lysine-specific demethylase 5C is a histone demethylase that specifically targets trimethylated and dimethylated, but not monomethylated, Lys-4 of histone H3, making it a regulator of the histone code. Lysine-specific demethylase 5C is involved in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements and is associated with Mental retardation, X-linked, syndromic, Claes-Jensen type (MRXSCJ). Lysine-specific demethylase 5C is widely expressed with highest levels found in brain and skeletal muscle. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect JARID using this rabbit polyclonal antibody, Anti-Lysine-specific demethylase 5C Antibody validated for use in western blotting, IP & IHC. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Lysine-specific demethylase 5C in 10 µg of human hippocampus tissue lysate. Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Lysine-specific demethylase 5C in human stomach tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a region near the near the N-terminus of human Lysine-specific demethylase 5C. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody is expected to react with all four known isoforms based upon immunogen design. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~170 kDa observed. Uniprot describes four isoforms at 176 kDa (isoform 1), 175 kDa (isoform 2), 171 kDa (isoform 3), and 155 kDa (isoform 4). Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human hippocampus tissue lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected Lysine-specific demethylase 5C in 10 µg of human hippocampus tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |