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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE498 |
| Description | Anti-Lysine-specific demethylase 7 Antibody |
| Alternate Names |
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| Background Information | Lysine-specific demethylase 7, also known as JmjC domain-containing histone demethylation protein 1D, and encoded by the gene JHDM1D/KDM7/KIAA1718, is an important histone demethylase particularly for brain development. JHDM1D specifically demethylates dimethylated Lys-9 and Lys-27 (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 Lys-20 residue (H4K20Me1), thereby playing a central role in histone code. JHDM1D also specifically binds trimethylated Lys-4 of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. JHDM1D also demethylates H3K9me2 in absence of H3K4me3 and JHDM1D has activity toward H4K20Me1 only when the nucleosome is used as a substrate and when not the histone octamer is used as substrate. JHDM1D is located in the nucleus and widely expressed but lowered expression is observed in glial cells and lymphoid cells. JHDM1D demonstrates tumor suppression by regulating angiogenesis factors when overexpression under starvation conditions in certain cancers, thus JHDM1D is being examined as a potential anti-cancer target. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Lysine-specific demethylase 7 using this rabbit polyclonal antibody, Anti-Lysine-specific demethylase 7 Antibody validated for use in western blotting. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Lysine-specific demethylase 7. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody is expected to cross react with two known isoforms of Lysine-specific demethylase 7 based upon immunogen design. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human and Mouse. Predicted to react with Canine, Mouse, Rhesus Macaque, Chimpanzee Giant Panda, and Marmoset based upon sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~110 kDa observed. Uniprot describes two isoforms at 107 kDa (isoform 1) and 92 kDa (isoform 2). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Lysine-specific demethylase 7 in 10 µg of HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |