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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P), IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE543 |
| Description | Anti-PRDM16 Antibody |
| Alternate Names |
|
| Background Information | Histone-lysine N-methyltransferase PRDM16 (UniProt: Q9HAZ2; also known as EC:2.1.1.367, PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1) is encoded by the PRDM16 (also known as KIAA 1675, MEL1, PFM13) gene (Gene ID: 63976) in human. PDRM16 is a transcription factor with 10 CeH2-type zinc finger domains. It is highly expressed in adipocytes where it is involved in browning and thermogenesis of adipocytes, the beigeing of adipocytes, the adipogenic differentiation of myoblasts, and the conversion of visceral adipocytes to subcutaneous adipocytes. Its expression is also observed in uterus, kidney, cardiomyocytes, and interstitial cells. It is reported to control a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. Loss of PRDM16 from brown fat precursors is reported to cause a loss of brown fat characteristics and promote muscle differentiation. Conversely, ectopic expression of PRDM16 in myoblasts is shown to induce their differentiation into brown fat cells. It stimulates brown adipogenesis by binding to PPAR- and activating its transcriptional function. PDRM16 also displays histone methyltransferase activity and monomethylates lysine 9 of histone H3 (H3K9me1) in the cytoplasm. It is also known to function as a repressor of TGF-β signaling. Four isoforms of PDRM16 have been described that are produced by alternative promoter usage and alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Takahata, M., et al. (2009). J. Biol. Chem. 284(5); 3334-3344; Seale, P., et al. (2008). Nature. 454(7207); 961-967). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PRDM16, Cat. No. ABE543, is a rabbit polyclonal antibody that detects PR domain zinc finger protein 16 (PRDM16) and is teste for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:200 dilution from a representative lot detected PRDM16 in Human kidney tissue sections. Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated PRDM16 in the nuclear extract of LNCap cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Histone-lysine N-methyltransferase PRDM16. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects Histone-lysine N-methyltransferase PRDM16. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Horse, Guinea Pig, Canine, Bovine, Non-Human Primate based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | Target molecular weight ~140 kDa observed; 140.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in nuclear extract of LNCap cells. Western Blotting Analysis: A 1:1000 dilution of this antibody detected PRDM16 in nuclear extract of LNCap cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |