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Millipore This Anti-S100a8/Mrp-8 Antibody (Azide Free) Is Validated For Use In Western Blotting And Immunohistochemistry And Activity Assay For The Detection Of S100a8/Mrp-8 (Azide Free). - Mill (Additional S&H or Hazmat Fees May Apply)
S100A8, also known as Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen (CFAG), Leukocyte L1 complex light chain, Migration inhibitory factor related protein 8 (MRP-8), p8, S100 calcium binding protein A8, or Urinary stone protein band A, and encoded by the gene S100A8/CAGA/CFAG/MRP8, is an important protein that binds both zinc and calcium and plays an important role in the regulation of inflammatory processes and immune response. Found in complex with its partner S100A9, S100A8 facilitates chemotaxis, fatty acid trafficking, cytoskeleton reorganization, and NAPDPH oxidase activation and other intracellular activities, as well as a host of extracellular induced activities such as extracellular proinflammatory, antimicrobial, oxidant scavenging, and apoptotic activities . Additionally S100A8 can act as a potent autoimmunity amplifier and appears to augment various cancers and their spread when over expressed. S100A8 is widely expressed and used as biomarker in patients with inflammatory diseases and as a cancer marker in multiple forms of cancer.
Product Information
Format
Serum
Presentation
Rabbit polyclonal serum without preservatives.
Quality Level
MQ100
Applications
Application
This Anti-S100A8/MRP-8 Antibody (Azide Free) is validated for use in Western Blotting and Immunohistochemistry and Activity Assay for the detection of S100A8/MRP-8 (Azide Free).
Key Applications
Western Blotting
Immunohistochemistry
Activity Assay
Application Notes
Activity Assay Analysis: A representative lot detected that S100A8/MRP-8 reduces mouse neutrophil accumulation in dorsum-raised air pouchs in the presence of MSU crystals (Ryckman, C., et Al. (2003). ARTHRITIS & RHEUMATISM. 48(8):2310–2320). Immunohistochemistry Analysis: A representative lot detected S100A8/MRP-8 in mouse arthritic paw tissue (Cesaro, A., et Al. (2012). PLOS ONE. 7(9):e4578).
Biological Information
Immunogen
Recombinant protein corresponding to mouse S100A8/MRP-8.
Host
Rabbit
Species Reactivity
Human
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_038678
Gene Symbol
S100a8
Caga
Mrp8
Purification Method
Unpurified
UniProt Number
P27005
Molecular Weight
~10 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human spleen tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected S100A8/MRP-8 in 10 µg of human spleen tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
